Activities of reactive oxygen species and superoxide dismutase in human lung adenocarcinoma cells impacted by moxa smoke solution

doi:10.3969/j.issn.2095-4344.2015.07.014

Abstract

Abstract:

BACKGROUND: The safety study of moxa smoke has been the focus of attention in the moxibustion research in recent years. As an important direction, the effects of moxa smoke on the human organ, tissue and cells have been discussed. Studies have shown that the solution of moxa smoke can induce apoptosis of human lung adenocarcinoma cells (A549), but the specific mechanism is still unclear.

OBJECTIVE: To observe the influences of moxa smoke on growth inhibition, intracellular reactive oxygen species and superoxide dismutase of A549 cells, and to explore the oxidative damage mechanism of moxa smoke on A549 cells.

METHODS: Moxa smoke was collected by a portable air sampler, and dissolved in DMEM to different concentrations, as 0.5, 0.4, 0.3, 0.2, 0.1, 0.05, 0.025, 0.01 g/L, which was used to culture A549 cells. Cell Counting Kit-8 method was employed to test cell growth inhibition rate, 2’,7’-Dichlorofluorescin diacetate method was employed to determinate reactive oxygen species intensity, and spectrophotometric method was employed to measure and calculate enzymatic activity of superoxide dismutase.

RESULTS AND CONCLUSION: The results of different concentrations of smoke solution intervening A549 cells in 4, 6 and 8 hours showed that the concentration of 0.4 g/L and 0.5 g/L on A549 cells growth inhibition rate was higher than 0.01 g/L and 0.025 g/L (P < 0.05). Low concentration of moxa smoke (0.05 g/L and 0.1 g/L) could decrease the intracellular levels of reactive oxygen species, but there was no statistically significant difference between them (P > 0.05); high concentration of moxa smoke (0.4 g/L) could increase intracellular levels of reactive oxygen species (P < 0.01), and decrease the activity of superoxide dismutase (P < 0.01). Moxa smoke is shown to have biological activity on A549 cells, and the mechanism of high-concentration moxa smoke effect on cell growth inhibition may be attributed to peroxidation.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Tissue Engineering, Safety, Cells

CLC Number:

R318

Dou Chuan-zi, Wu Huan-gan, Wang Shuo-shuo2, Ma Xiao-peng, Huang Yan, Zhao Ji-meng, Hu Zhi-hai, Liu Hui-rong, Cui Yun-hua, Zhou Ci-li, Zhao Chen. Activities of reactive oxygen species and superoxide dismutase in human lung adenocarcinoma cells impacted by moxa smoke solution[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(7): 1057-1062.

Figures/Tables 4

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